|
|
|
Waikato DNA Sequencing Facility
|
GS Junior Services
An optimized solution for a wide variety of sequencing applications.
De Novo Sequencing
Sequence complete bacterial, viral, small fungal and other small genomes or multiple BACs and plasmids on the GS Junior System. Use long single reads alone, or in combination with paired end reads to generate high-quality de novo assemblies with few contigs. Perform comparative analysis of multiple whole genomes and identify differences between samples.
Targeted Resequencing
Perform ultra-deep sequencing of amplicons (PCR products) with the GS Junior System. Generating an average of 100,000 high quality reads per run, the system is ideally suited for detecting low level variants (<1% frequency) in pooled amplicon samples, such as viral or human DNA. For many targeted sequencing projects, dozens or hundreds of amplicons can be tiled across genomic regions of interest (i.e. disease-associated regions) and sequenced together.
Targeted sequencing using sample-enrichment technologies
Sequence capture technologies allow researchers to target and sequence only the regions of interest in the genome and assess the genetic variation within the captured DNA regions. The GS Junior System is ideal for small sequence capture projects that require sequencing of 40 million bases or less.
Metagenomic Characterization
The GS Junior System enables a comprehensive view into the diversity and metabolic profile of an complex environmental samples. Analysis of 100,000 reads can yield a high percentage of species identification in complex metagenomes and even higher in less complex samples. The long reads provide the enormous specificity needed to compare sequenced reads against DNA or protein databases and give unambiguous assignment of even closely related species.
|
|
|
| |
|
Faculty of Science and Engineering - Te Mātauranga Pūtaiao me te Pūkaha
The University of Waikato - Te Whare Wananga o Waikato
Last modified: Mon Feb 14 10:38:00 2011
Page Generated: Thu Feb 23 20:52:23 2012
URL: http://bio.waikato.ac.nz/sequence/454.shtml
This page has been reformatted for printing
|
