Department of Biological Sciences

Waikato DNA Sequencing Facility

SNP Typing Services

The WDSF operates a SNP typing service based on the SNuPe SNP Profiling system. The SNuPe reaction extends the primer by a single nucleotide, adding a fluorescently labelled reporter to the SNP site for subsequent detection.

Template

The template requirements for SNP typing match those of the sequencing service excluding that only PCR based templates can be tested.

Primers

SNP testing requires only a single unlabelled primer specifically designed to be a single base upstream to the SNP of interest.

• SNP typing requires primers at a concentration of 1nM/uL (1 micro molar)
• SNP primers must bind so that the SNP position is the base immediately downstream of the binding location

Availability

Due to the nature of the SNP typing service, the minimum number of reactions or run only samples we would prefer to run is 16.