Department of Biological Sciences

Waikato DNA Sequencing Facility

Genotyping Services

The Waikato DNA sequencing facility operates a Genotyping service based on the 3130 Genetic Analyser and GeneMapper 4 series software. The DNA template requirements for genotyping match those of the sequencing service in terms of required concentrations and template purity. Genotyping requires one primer from each pair to be labelled with a suitable reporter.

Genotyping Ranges

The fragment profiling system is capable of accurately differentiating genotypic fragments up to 1200bp in length in two distinct ranges according to the standard. The WDSF can supply standards for 600 or 1200 bp runs.

Fluorescent Reporters

Each primer pair requires that one primer of the pair is labelled with a fluorescent reporter, the availability of certain reporters may depend on your chosen primer supplier. The WDSF can accept and utilises the following reporters.

ABI G5(DS-33) Dye Set; FAM, VIC, NED, PET and LIZ
Promega PowerPlex 5-Dye; FL, JOE, TMR, CXR and CC5

Results

Results are available as either a Fragment List (listing of all fragments) or a called List with results sorted and counted (binned) according to customer specified product lengths.

Availability

Due to the array nature of the genotyping service, we prefer to run at least 16 samples at a time.